Estimation of Subcellular Proteomes in Bacterial Species

Estimation of Subcellular Proteomes in Bacterial Species

Computational methods for predicting the subcellular localization of bacterial proteins play a crucial role in the ongoing efforts to annotate the function of these proteins and to suggest potential drug targets. These methods, used in combination with other experimental and computational methods, can play an important role in biomedical research by annotating the proteomes of a wide variety of...

An Empirical Strategy for Characterizing Bacterial Proteomes across Species in the Absence of Genomic Sequences

Global protein identification through current proteomics methods typically depends on the availability of sequenced genomes. In spite of increasingly high throughput sequencing technologies, this information is not available for every microorganism and rarely available for entire microbial communities. Nevertheless, the protein-level homology that exists between related bacteria makes it possib...

Comparative Study of L-asparaginase Activity in Different Bacterial Species

Genome update: prediction of secreted proteins in 225 bacterial proteomes.

The Bacteroides fragilis NCTC 9343 genome is the fifth member of the Bacteroidetes/ Chlorobi group to be sequenced and the second Bacteroides fragilis strain. The Bacteroides fragilis NCTC 9343 genome shows considerable variation even within the same strain and ‘invertable promoters’ appear to regulate much of this variance in many surface-exposed and secreted proteins (Cerdeño-Tárraga et al., ...

Coevolution of DNA Uptake Sequences and Bacterial Proteomes

Dramatic examples of repeated sequences occur in the genomes of some naturally competent bacteria, which contain hundreds or thousands of copies of short motifs called DNA uptake signal sequences. Here, we analyze the evolutionary interactions between coding-region uptake sequences and the proteomes of Haemophilus influenzae, Actinobacillus pleuropneumoniae, and Neisseria meningitidis. In all t...